PMC

Concatenated sequences of core genes used in this analysis: Iridovirus core gene 2 (EHNV 7R)—RNApol II, a subunit; Iridovirus core gene 3 (EHNV 8L)—NTPase/ helicase; Iridovirus core gene 4 (EHNV 10L)—RAD2; Iridovirus core gene 5 (EHNV 11R)—unknown function; Iridovirus core gene 7 (EHNV 14L)—MCP; Iridovirus core gene 8 (EHNV 16L)—thiol oxidoreductase; Iridovirus core gene 9 (EHNV 18L)—deoxynucleoside kinase; Iridovirus core gene 12 (EHNV 24R)—RNAse III; Iridovirus core gene 13 (EHNV 38R)—ribonucleotide reductase, small subunit; Iridovirus core gene 14 (EHNV 43R)—RNApol II, b subunit; Iridovirus core gene 15 (EHNV 44L)—DNApol; Iridovirus core gene 17 (EHNV 53L)—myristylated membrane protein; Iridovirus core gene 19 (EHNV 72R)—unknown function; Iridovirus core gene 21 (EHNV 85L)—D5 NTPase; Iridovirus core gene 22 (EHNV 86R)—unknown function; Iridovirus core gene 23 (EHNV 89L)—serine/ threonine protein kinase; Iridovirus core gene 24 (EHNV 92L)—NTPase. Numbers at the nodes of the tree indicate MrBayes posterior probabilities of 1.000.000 replicates. Lymphocystis disease virus China (LCDV-C) was used as an outgroup. Classifications of the viruses to the different ALRV-groups based on their genomic arrangement are indicated beside the brackets. GenBank accession numbers of the sequences from ALRV used in the analysis are given in ; EHNV(FJ433873), ESV(JQ724856), grouper iridovirus (GIV) (AY666015), Singapore grouper iridovirus (SGIV) (AY521625), LCDV-C (AY380826).

Phylogenetic analysis of iridoviruses. Maximum-likelihood analysis of 26 core gene amino acid sequence concatenates (8178 aa). The tree is rooted at the midpoint. Bootstrap values are given as percentages. ADRV, Andrias davidianus ranavirus; AMIV, Anopheles minimus iridovirus; ATV, Ambystoma tigrinum virus; BIV, Bohle iridovirus; CH, Testudo hermanni ranavirus; CMTV, common midwife toad virus; CoIV, cod iridovirus; DFV, doctor fish virus; ECV, European catfish virus; EHNV, epizootic haematopoietic necrosis virus; ESV, European sheatfish virus; FV, frog virus; GGRV, German gecko ranavirus; GIV, grouper iridovirus; GSIV, giant seaperch iridovirus; GV, guppy virus; IIV, invertebrate iridescent virus; ISKNV, infectious skin and kidney necrosis virus; LCDV, lymphocystis disease virus; LMBV, largemouth bass virus; LYCIV, lemon yellow croaker iridovirus; OSGIV, orange-spotted grouper iridovirus; PPIV, pike perch iridovirus; RBIV, rock bream iridovirus; RGV, Rana grylio virus; Rmax, Rana maxima virus; RSIV, red seabream iridovirus; SDDV, scale drop disease virus; SERV, short-finned eel ranavirus; SGIV, Singapore grouper iridovirus; SSME, spotted salamander Maine virus; STIV, soft-shelled turtle iridovirus; TFV, tiger frog virus; ToRV, tortoise ranavirus; TRBIV, turbot reddish body iridovirus

Phylogenetic trees of iridoviruses. (A) Alignment of the genus Iridovirus based upon a partial major capsid protein sequence as described in the work of Webby and Kalmakoff (). The Chloriridovirus IIV-3 and the Lymphocystivirus LCDV-1 are included. (B) Alignment of representatives of the five iridovirus genera Chloriridovirus (IIV-3), Iridovirus (IIV-6, IIV-9), Lymphocystivirus (LCDV-1), Ranavirus (ISKNV), and Megalocytivirus (SGIV) based upon a concatenated amino acid sequence of the 26 core iridovirus genes. Bootstrap support from 1,000 iterations is indicated for all branches, with at least 70% support.

Northern blot hybridization against grouper iridovirus caspase recruitment domain (GIV-CARD) mRNA in GIV-infected GK cells at various time points after GIV infection (10 m.o.i.), or in the presence of cycloheximide (CHX) or aphidicolin (APH). Blots against 18 s rRNA are shown for each sample to represent total RNA quality and quantity. C, control, no GIV infection.